Journal: International Journal of Molecular Sciences

Article Title: Inhibition of Amyloid-β (Aβ)-Induced Cognitive Impairment and Neuroinflammation in CHI3L1 Knockout Mice through Downregulation of ERK-PTX3 Pathway

doi: 10.3390/ijms25105550

Figure Lengend Snippet: CHI3L1 related to AD. Human serum analysis of Aβ 1–40 , Aβ 1–42 , Tau, and CHI3L1 in patients with AD and healthy controls using ELISA ( n = 25). The ( A ) serum levels and ( B ) ROC curves of Aβ 1–40 , Aβ 1–42 , Tau, and CHI3L1. ( C ) Spearman correlation test results between CHI3L1 and Aβ 1–40 , Aβ 1–42 , or Tau. Each value is mean ± S.E.M. from 25 samples. *, Significantly different from control group ( p < 0.05). ***, Significantly different from control group ( p < 0.001). n.s., not significant.

Article Snippet: Aβ 1–42 and Aβ 1–40 levels were determined using each specific mouse Aβ 1–42 enzyme-linked immunosorbent assay (ELISA) Kit and mouse Aβ 1–40 ELISA Kit purchased from CUSABIO (Houston, TX, USA) following the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Acta Neuropathologica

Article Title: Cerebral hypoperfusion accelerates cerebral amyloid angiopathy and promotes cortical microinfarcts

doi: 10.1007/s00401-011-0925-9

Figure Lengend Snippet: Enhanced Aβ deposition in Tg-SwDI mice after cerebral hypoperfusion. a Aβ immunohistochemical staining showing that following cerebral hypoperfusion, Tg-SwDI mice exhibited cortical Aβ deposition at 24 weeks of age ( right middle panel ) and more extensively at 32 weeks of age ( right lower panel ). Sham-operated Tg-SwDI mice showed cortical Aβ deposition at 32 weeks of age ( left lower panel ). Insets show representative images of vascular and perivascular Aβ deposition. b Aβ immunohistochemical staining showing that following cerebral hypoperfusion, Tg-SwDI mice exhibited Aβ deposition in the leptomeninges containing leptomeningeal arteries ( arrows ) at 18 weeks of age ( right upper panel ) and more extensively at 24 and 32 weeks of age ( right middle and lower panels ), although sham-operated Tg-SwDI mice began to show such changes at 32 weeks of age ( left lower panel ). Insets show representative images of vascular and perivascular Aβ deposition in the leptomeningeal artery. In a and b , the mouse anti-Aβ 1–40 (BA27) antibody was used. c The schematic illustration of the regions of interest depicted in the cerebral cortex (size, 500 × 300 μm each), the hippocampus (500 × 300 μm each), and the leptomeninges including the pia and arteries (300 × 50 μm each) of the parasagittal section of the brain. d – f Densitometric analyses of temporal profiles of Aβ depositions in the cerebral cortex ( d ), the hippocampus ( e ), and the leptomeninges ( f ). Leptomeningeal vascular, as well as pial, Aβ accumulation was jointly analyzed as ‘leptomeningeal Aβ’. There seems to be a ceiling effect of age on leptomeningeal Aβ accumulation. CPu caudoputamen, Cx cortex, Hip hippocampus, LM leptomeninge, LV lateral ventricle, Thal thalamus. Bars indicate 40 μm in a and b and 8 μm in insets in a and b

Article Snippet: Mouse anti-Aβ 1–40 (BA27) (Wako amyloid kit, Wako, Osaka, Japan), mouse anti-Aβ 1–42 (BC05) (Wako amyloid kit, Wako), and mouse anti-Aβ 5–10 (6E10) (1:500, Covance, Princeton, NJ, USA) antibodies were used.

Techniques: Immunohistochemical staining, Staining